All articles, part 1: http://theorbital.ru/files/2017/09/Orbital-1.pdf
Startseva O., Paliy I., Simagina N., Makaricheva A.
FAST SEMI-QUANTITATIVE METHOD OF DETERMINATION OF CHLOROGENIC ACID AND ITS DERIVATIVES IN SAGEBRUSH
Abstract. Fast semi-quantitative method of determination of chlorogenic acid and its derivatives in sagebrush has been developed. Two wild-growing Crimean sagebrush species have been analyzed. The efficiency of this method to estimate the content of these biologically active compounds aiming to their further separation from herbal raw material has been shown.
Keywords: chromatography, phenolics, chlorogenic acid, di-O-caffeoylquinic acids, herbal raw material.
Gasparyan A.K., Nekhoroshev S.V., Nekhoroshev V.P., Knyazev A.S., Kakova D.A.
SYNTHESIS OF PYROMELLITEIN INDICATORS BASED ON PYROMELLITIC DIANHYDRIDE
Abstract. The exploratory research has been carried out towards the synthesis of pyromellitein indicators employing the condensation between PMDA with different phenols. The optimal synthetic conditions were found, using ZnCl2 as a catalyst within the reaction temperatures ranging from 120 to 210 °C and a contact time of 3 to 6 hours. The yield of pyromellitein indicators ranged from 20 to 80%. The target compounds must be purified from impurities. The structures of 7 pyromellitein indicators were confirmed by UV and IR spectroscopy, HPLC / MS and DSC.
Keywords: synthesis of pyromellitein indicators, condensation reaction, optimal synthetic conditions, IR spectrometry, differential scanning calorimetry.
Grebennikova O.A., Paliy A.Y., Khlypenko L.A., Rabotyagov V.D.
BIOLOGICALLY ACTIVE SUBSTANCES OF Hyssopus officinalis L.
Abstract. This paper presents the data on qualitative and quantitative composition of a number of biologically active substances (volatile compounds, phenolic compounds and vitamins) in aqueous-ethanolic extract from Hyssopus officinalis L. Nikitskiy Belyiy variety bred in Nikitsky Botanical Garden — National Scientific Center (hereafter referred to as NBS–NSC). The concentration of volatile compounds in the aqueous-ethanolic extract of common hyssop is about 600 mg/100 g. 38 components have been determined in the extract, 32 of them have been identified. It has been found that monoterpene ketones (81.6 % (rel.)) and monoterpenes (7.3 % (rel.)) predominate among volatile substances. The main volatile compounds of the common hyssop extract are pinocampone, iso-pinocampone and β-pinene. The total phenolics content in the aqueous-ethanolic extract of the common hyssop was 592.1 mg/100 g. 17 components have been determined in the extract. Rosmarinic acid (169.2 mg/100 g) dominates among phenolic compounds of the common hyssop extract. The content of ascorbic acid (14,54 mg/100 g) and carotenoids (5,19 mg/100 g) has been quantified. A conclusion about the possibility of using the extract for developing food, therapeutic and prophylactic products is made.
Keywords: medicinal herbs, Hyssopus officinalis L., aqueous-ethanolic extract, chromatography, mass spectrometry, volatile compounds, phenolic compounds, vitamins.
Airapetov M.I., Eresko S.O.
DYNAMICS OF VARIATION OF mRNA AMOUNT OF CRF-R2 RECEPTOR IN HIPPOCAMPUS OF CHRONICALLY ALCOHOLIZED RATS UNDER ALCOHOL WITHDRAWAL
Abstract. Chronic alcoholic intoxication is characterized by the decreasing control on the amount of alcohol intake as well as increasing tolerance towards it. It is known, that corticoliberine receptors of the second type (CRF-R2) are involved in the mechanism of chronic alcoholic intoxication. It was shown, that CRF-R2 are localized in hippocampus. In the course of our investigation we estimated the variation character of amount of mRNA CRF-R2 in hippocampus during abstinence syndrome. The obtained data show, that the amount of mRNA corticoliberine receptors of the second type in hippocampuses of chronically alcoholized rats during the alcohol withdrawal period has tendency for increasing at first and seventh days. Our results demonstrate dysregulation in neuromittors exchange in hippocampus area, which allows us to assume, the formation of cognitive impairment in the organism, which leads to the reduction of control on the amount of alcohol consumption.
Key words: corticoliberine receptors, mRNA, alcoholism, hippocampus.
INFLUENCE OF AGONIST AND ANTAGONIST FOR D2-TYPE DOPAMINERGIC RECEPTORS ON AVOIDANCE PERFORMANCE IN RATS WITH DIFFERENT ANDROGENS LEVELS
Abstract. This research work represents a comparative analysis for agonist and antagonist of D2-types dopaminergic receptors effects on passive avoidance performance in intact, gonadectomized (GDX) and GDX male rats administered by testosterone propionate. The effects for chronic administration of agonist of D2-type dopaminergic receptors — quinperole (0,1 mg/kg, i.p.) and antagonist of D2-type dopaminergic receptors — sulpiride (10,0 mg/kg, i.p.), as well as its combination with a low dose of testosterone propionate (0,5 mg/kg, s.c.) were tested during 14 days on behavioral processes in the passive avoidance paradigm and the «open field» test. Simultaneously, we measured testosterone levels in the blood serum using test-system in vitro by immune-enzyme assay. Co-administration of quinperole with a low dose of testosterone propionate completely restored impaired passive avoidance performance in GDX males. Moreover, GDX rats were treated with quinperole plus a low dose of testosterone propionate and demonstrated increased exploratory and grooming behavior in the «open field» test. Both sulpiride alone and in combination with low dose of testosterone propionate markedly impaired passive avoidance learning in GDX rats. The results of the present study suggest that chronic administration of quinperole (stimulation of D2-type dopaminergic receptors) markedly improves passive avoidance performance in GDX rats. Moreover, co-treatment with quinperole and testosterone propionate completely corrects passive avoidance learning in GDX male rats. Hormonal assay showed that chronic administration of quinperole alone or in a combination with testosterone propionate corrected testosterone levels in the blood serum in GDX rats as compared to the control rats. However, chronic treatment with sulpiride (blockade of D2-type dopaminergic receptors) significantly impairs capability of GDX rats to formation of passive avoidance performance. Moreover, its combination with testosterone propionate resulted in blockade of positive effect for testosterone propionate on passive avoidance performance.
Keywords: quinperole, sulpiride, D2-type dopaminergic receptors, avoidance learning, behavior, androgens, gonadectomy.
INFLUENCE OF TYPE OF SUBSTRATE AND DURATION OF ITS PREPARATION ON RATE OF REPRODUCTION OF PLEUROTUS OSTREATUS MYCELIUM
Abstract. This paper presents the study of the influence of different substrate types on the basis of grains of cereal cultivars and the duration of their preparation on the rate of reproduction of the mycelium of Pleurotus ostreatus. The study was carried out in the school laboratory of Sheryinskaya secondary school at Nytvensky District of Perm krai. Oats were found to be the best substrate for reproduction of the mycelium of Pleurotus ostreatus. The highest rate of reproduction of the mycelium of Pleurotus ostreatus (70 %) was obtained on this substrate, due to its greater filminess.
The best way of substrate preparation is its boiling (disinfection) for 30 min.
Key words: Pleurotus ostreatus, type of substrate, grain, mycelium, rate of reproduction.